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A highly clonogenic subset of adult mouse biliary duct cells

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE73897
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Our goal is to find the heterogeneity of mouse liver ductal cells in the wild type mouse liver. We separated the MIC1-1C3+ mouse liver ductal cells into two populations with the cell surface marker: ST14. We found that ST14hi1C3+ cells have higher colonal geneity comparing to the ST14lo1C3+ population. Mouse liver NPCs were sorted by flowcytometry. The MIC1-1C3+CD26- non blood cells are seperated into two parts: ST14high and ST14low. Fresh sorted cells are put into TRIzol for RNA. RNA seq libraries were made with illumina TruSeq kit under the manufacture's protocol.
创建时间:
2019-05-15
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