Nucleotide metabolism-related host proteins RPB2 and UPP1 interacting with PEDV N proteins affect viral replication

Porcine epidemic diarrhea virus (PEDV) is a highly pathogenic infectious disease targeting pig intestine that is widespread in the world, causing huge economic losses to pig industry. PEDV N protein is the core protein of PEDV, which locates in the cytoplasm and wraps the RNA genome of the virus to form spiral ribonucleoprotein (RNP). However, host proteins interacting with N proteins and their role in PEDV replication have not been fully elucidated. In this study, PEDV-N labeled antibodies were immunoprecipitated (Co-IP) and combined with LC-MS/MS to screen host proteins interacting with N proteins.