Quantifying intraspecific variation in host resistance and tolerance to a lethal pathogen

Testing for intraspecific variation for host tolerance or resistance in wild populations is important for informing conservation decisions about captive breeding, translocation, and disease treatment. Here, we test the importance of tolerance and resistance in multiple populations of boreal toads (Anaxyrus boreas boreas) against Batrachochytrium dendrobatidis (Bd), the amphibian fungal pathogen responsible for the greatest host biodiversity loss due to disease.
Boreal toads have severely declined in Colorado (CO) due to Bd, but toad populations challenged with Bd in western Wyoming (WY) appear to be less affected. We used a common garden infection experiment to expose post-metamorphic toads sourced from four populations (2 in CO and 2 in WY) to Bd and monitored changes in mass, pathogen burden, and survival for eight weeks. We used a multi-state modeling approach to estimate weekly survival and transition probabilities between infected and cleared states, reflecting a dynamic infection ..., See main manuscript text and supporting information in Hardy et al., , # Quantifying intraspecific variation in host resistance and tolerance to a lethal pathogen

[https://doi.org/10.5061/dryad.0rxwdbs82](https://doi.org/10.5061/dryad.0rxwdbs82)

The ‘Multistate_Datafile’ is a file formatted in the multi-state mark-recapture format for program MARK.

### Description of Multistate\_Datafile

*Filler_1:* /* required before individual identifier (id) entry for MARK software to separate individuals

*id:* individual animal identity

*Filler_2:* */ required after individual identifier (id) entry for MARK software to separate individuals

*Day0:* Entry of an I (infected), C (cleared/not infected), or 0 (dead) to signify whether an individual was infected with *Batrachochytrium dendrobatidis*, not infected, or dead on the day of sampling.

*Day7:* Entry of an I (infected), C (cleared/not infected), or 0 (dead) to signify whether an individual was infected with *Batrachochytrium dendrobatidis*, not infected, or dead on the day of sampling.

*Day14:* Entry of an ...