Transciption profiling of leptin receptor-expressing neurons in the Nucleus of the Solitary Tract

This study aims to identify and compare unique transcription profiles of neurons in the NTS that express the leptin receptor. We conducted single-cell patch RNA-sequencing experiments to classify neurons first by their unique firing properties and, second, transciptional profiles. We found two unique cellular populations: type1 and type2 distinguishable by firing patterns and transciption profile. Overall design: cytoplasmic and nuclear RNA were harvested from single-neurons of transgenic mice with yellow flourescent protein expressed in leptin receptor-expressin neurons (LepRb-Cre; Halo-eYFP)