KYSE (450, 520, 890, 960) cell lines
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下载链接：
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE25464
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资源简介：
microRNA profiles in human esophageal squamous cell carcinoma cell lines KYSE cell lines were maintained in Ham’s F-12/RPMI 1640 (Invitrogen, San Diego, CA) containing 5% fetal bovine serum at 37 ˚C in a fully-humidified 5% CO2 atmosphere. Total RNA was extracted by the acid guanidinium thiocyanate-phenol-chloroform method. According to manufacture’s instructions, 500 ng of total RNA was labeled with Hy5 and was hybridized on the Human miRNA Oligo chip for 16 hour at 32˚C. A Human miRNA Oligo chip was scanned using ProScanArray (PerkinElmer).
创建时间：
2016-11-06
