five

A directory of primers used to analyze different splice variants of ANTXR1 (TEM8).

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Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_A_directory_of_primers_used_to_analyze_different_splice_variants_of_ANTXR1_TEM8_/261339
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S, sense; AS, antisense. 1°, primary PCR. 2°, secondary (nested) PCR. The 5′ sense primers S3 and S21 are common to all TEM8 variants. The pairs of sense and antisense primers for primary and nested PCR had partial overlaps. V2-as1251 and V2-as1273 are 30 nucleotides long. All other primers are 27 nucleotides long. The expected amplicons after 2° PCR are: V1, 1848 bp (with as1842); V2, 1260 bp; and V3, 1358 bp.
创建时间:
2015-12-02
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