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ChIP seq of mESC treated with doxorubicin

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https://www.ncbi.nlm.nih.gov/sra/SRP310177
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资源简介:
mESC were treated with doxorubicin and then histone modifications or p53 binding sites were analyzed Overall design: mESC were treated with doxorubicin 1uM or vehicle for 6 hours, washed out for 6 hours, then crosslinked with 1% formaldehyde at RT for 10 min, quenched with 0.125M glycine for 10 min, then snap frozen in liq N2. ChIP was performed as described in Rada-Iglesias, et al. Nature 2010.
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2021-05-23
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