Transcriptional alteration in malaria parasite after disruption of PfPRMT5 [Array]

Purpose: this study is to analyze the change of overall transctiption after disruption of Protein Arginine Methyltranferase 5 (PRMT5) in Plasmodium falciparum. Methods: In this study, the transcriptomes of a PfPRMT5 gene knockout (KO) parasite line with its wildtype control were analyzed a custom-desinged Agilent microarray.Total RNA were harvested from the asexual parasites at four stages (ring, early trophozoite, late trophozoite, and schizont)(12h, 24h, 36h, and 46h post-invasion) using the Quick-RNA MiniPrep kit (Zymo Research). A three replicates of 8-chip study using total RNA recovered from a PfPRMT5 gene knockout (KO) parasite line with its wildtype control at ring (12h), early trophozoite (24h), late trophozoite (36) and schizont (46) stage. The chip used in this stury was a custom designed microarray by agilent eArray (array name: MALARIAARRAY_JM, Design ID: 048532, Design format: 4 X 44K, Control grid: IS-45220-4-V1_4x44K_GX_EQC_V20060608)