PlexinA4-Semaphorin3A mediated crosstalk between main cortical interneuron classes is required for superficial interneurons lamination

We demonstrate that the two main classes of interneurons (INs) communicate during cortical development. MGE-derived INs first colonize deep layers and serve as guidepost for later-arriving HTR3A+ INs. This process relies on PlexinA4 and Semaphorin3A signaling and is critical for the proper distribution of inhibitory neurons across layers.
Data here is files containing manual counting of neurons in the mouse somatosensory cortex at different ages (details are included in each of them). Data in 3 contains area measurements of growth cones after embryonic dissociated cell cultures. Data in 4 and 6 contains dynamic tracking of cells after time-lapse imaging. For each excel file, an associated prism file contains related statistical analyses.

本研究证实，在皮层发育过程中，内神经元（INs）的两大主要类别相互沟通。起源于MGE的内神经元首先占据深层区域，并为随后到达的HTR3A+内神经元提供导向。此过程依赖于PlexinA4和Semaphorin3A信号传导，对于抑制性神经元在各个层次上的恰当分布至关重要。所提供数据包括在不同年龄阶段小鼠躯体感觉皮层中神经元的手动计数文件（详细情况包含在各个文件中）。数据3包含胚胎解离细胞培养后生长锥的面积测量。数据4和6包含细胞在时间推移成像后的动态追踪。每个Excel文件均附带一个相关棱镜文件，其中包含相关的统计分析。