Transcriptome analysis of pancreatic tumor cell invasion and angiogenesis in the PDAC-CAM model. Transcriptome analysis of pancreatic tumor cell invasion and angiogenesis in the PDAC-CAM model

Human pancreatic adenocarcinoma cells were grafted on the chick chorioallantoic membrane (CAM). Human and chicken GeneChips were used simultaneously to study gene regulation during PDAC cell invasion. Overall design: Pooled RNA from T1 (n=3) and T6 (n=3) CAMs were extracted using the RNeasy mini kit (Qiagen). T6 was compared to T1 on human GeneChips and in parallel, on chicken GeneChips.