Rate of various tissue defects compared between Tape-Transfer sectioned material and published images from the Allen Reference Atlas.

Most of the categories are self-explanatory. Those that are not, we define as such: "Knife Damage" refers to torn sections that display a periodic "window blind" like pattern parallel to the knife. "Entire Section Missing" refers to instances where the section was intended to be collected, but did not adhere to the slide. “Thermal Damage” refers to non-anatomical holes that can appear in the tissue due to a rapid change in Temperature of the sections during cutting. Whole-brain thermal damage can also be caused due to improper cryo-protection of the sample during preparation. For the Allen Reference Atlas brains, only the sections shown in the atlas have been evaluated. For the coronal brain, these represent ¼ of the collected 25μm sections (~100μm spacing). Scale bars are 5mm.
Rate of various tissue defects compared between Tape-Transfer sectioned material and published images from the Allen Reference Atlas.