Ratio of dead cells as compared to untreated cells after TRAIL treatment.

Conditioned medium from TRAIL-producing P. pastoris clones was 100-fold diluted in DMEM/FBS. Subconfluent prostate carcinoma PPC-1 in a 96-well plate (100 μl/well) were incubated with 1 and 3 μl of the diluted TRAIL samples for 24 h. At the end of the treatment, the ratio of dead cells was determined by an ATPLite reagent. The conditioned medium obtained from non-transfected yeast cells was not cytotoxic to PPC-1 cells. The cytotoxic activity of the most efficient clones for each TRAIL construct is shown.Ratio of dead cells as compared to untreated cells after TRAIL treatment.