RNA-seq analysis of synovial fibroblasts isolated from Ets1-flox/flox and Col6a1-Cre-Ets1-flox/flox mice of serum transfer-induced arthritis (STIA)

Synovial fibroblasts critically contribute to the pathogenesis of rheumatoid arthritis (RA) by acquiring either a pro-inflammatory or tissue-destructive phenotype. To explore the molecular mechanisms underlying the tissue-destructive fibroblast phenotype in arthritis, we performed bulk RNA-sequencing analysis on the synovial fibroblasts which were isolated from Col6a1-Cre-Ets1-flox/flox (Ets1ΔFib) and Ets1-flox/flox (Ets1flox) mice. RNA-seq analysis was performed on synovial fibroblasts (CD45-CD31-CD146-PDPN+) isolated from the ankle joints of Ets1flox and Ets1ΔFib mice under STIA condition (n=3 per group) . Data were acquired on an Ion Proton sequencer (Thermo Fisher Scientific) and CLC genomics Workbench v.12.