Border-like cells production mediated by SgPG1 proclaims aluminum resistance in Stylosanthes guianensis

In order to investigate molecular mechanism underlying Reyan No. 2 BLCs development, transcriptomic analysis was conducted in Reyan No. 2 root tips at 24 and 48 h after germination. For each time course, three libraries were constructed and produced around 50 and 52 million raw reads, and 40.5 and 45.5 million clean reads, respectively. A total of 2036 genes, which were involved in various biological processes, such as the cell wall modification, exhibited differential expression patterns between 48 h and 24 h after germination. Among these differential expression genes (DEGs), 467 genes were up-regulated, while 1569 genes were down-regulated. Furthermore, a total of 90 DEGs were predicted to be involved in cell wall modification, with 16 genes up-regulated and 74 genes down-regulated. The qRT-PCR assay was further conducted to investigate the expression levels of 12 randomly selected DEGs, and results were consistent with the assay of transcriptomic analysis Overall design: transcriptomic analysis was conducted in Reyan No. 2 root tips at 24, 48 and 72h after germination