Impact of NLUCAT1 deletion on A549 cells in normoxic and hypoxic conditions

Three independent parental or NLUCAT1 CRISPR/Cas9-deleted A549 clones were cultured in normoxia (Nx) or exposed to hypoxia (Hx) at 1% O2 for 24 hours. Experiments were performed in a one color design, corresponding to 4 conditions performed in triplicates (WT A549 Nx, n = 3 clones; WT A549 Hx, n = 3 clones; DEL A549 Nx, n = 3 clones, DEL A549 Hx, n = 3 clones) for a total of 12 samples.