The effects of microRNA-22 loss in the megakaryocytic and erythrocytic lineages

The goal of this study was to identify genes dysregulated upon modulation of microRNA-22 expression in the megakaryocytic and erythrocytic hematopoietic lineages. These goals were achieved through RNA-sequencing analysis of multiple samples: megakaryocyte-erythroid progenitors (MEP) isolated from microRNA-22 knockout mice, the K562 human erythroleukemia cell line knocked-out for microRNA-22 by CRISPR/cas9 genome editing, and K562 cells overexpressing miR-22 through transient transfection.