RNA expression in P1 sciatic nerves

RNA seq was performed comparing SC-specific Dicer mutants with SC-specific Lin28B transgenics to obtain an unbiased list of potentially de-regulated miRNA target candidates. total RNA from sciatic nerves of P1 Dicer mutants, P1 Lin28 transgenics and their respective controls was used to perform RNA sequencing analysis.本研究通过RNA测序（RNA-seq）技术，对比施万细胞（Schwann Cell, SC）特异性Dicer突变体与施万细胞特异性Lin28B转基因模型，以获取一组无偏倚的潜在失调微小RNA（microRNA, miRNA）靶标候选基因列表。实验取材于出生后第1天（P1）Dicer突变体、P1 Lin28转基因模型及其对应对照组的坐骨神经总RNA，并以此开展RNA测序分析。