The primary dipole of flipper probes

Table of contents

1. Synthesis and characterization

Compound 8

Compound 16

Compound 2

Compound 2’

Compound 10

Compound 18

Compound 3

Compound 3’

2. Fluorescence spectroscopy

Solvatochromism

Solvatochromism labbook

Absorbance

Fluorescence

Fluorescence quantum yields

Labbook fluorescence quantum yields

Fluorescein + 2-3-2'-3'

Quinine + 3' hydrate

Dynamic covalent ketone chemistry

Kinetics of hydrate formation

Water concentration dependence

Fluorescence spectroscopy in LUVs

Concentration dependence in LUVs

Partition kinetics

Partition coefficients

3. CLSM and FLIM

Confocal microscopy

FLIM

Data Format:

Most data are in open format.

Fluorescence data (OPJ) can be read in Origin Viewer (https://www.originlab.com/viewer/).

CLSM file (lif) can be read in FIJI/ImageJ (https://fiji.sc/).

FLIM data can be read in SymPhoTime software (Picoquant).

Compound Numbers and Codes Correlations:

1 = JG-1-54

1’ = JG-1-64

8 = JG-2-12

16 = JG-2-14

10 = JG-2-5 = JG-2-18

18 = JG-2-9

2 = JG-2-15

3 = JG-2-10

2’ = JG-2-17

3’ = JG-2-16