scRNA-Seq of immune populations from nasal mucosa and lungs of C57BL/6 mice and BLT1 receptor deficient mice infected intranasally with MCMV K181 strain

We used a single cell sequencing approach using 10x Genomics scRNAseq to understand the transcriptional differences between immune populations from MCMV infected wildtype C57BL/6 mice and BLT1-/- mice. IFNy producing cells were similar between both experimental groups, however, BLT1-/- mice showed a reduced contribution of IFNy from CD8+T cells which was sublte in the nasal mucosa (NM) but obvious in the lungs. Comparison of T cell clusters showed that WT T cells had increased gene signatures associated with proliferation, integrin signaling, cytoskeleton regulation by Rho GTPases, T cell activation, and cytokine/chemokine signaling compared to BLT1-/- T cells, with this being true in CD8 clusters in the NM and both Cd4 and CD8 in the lungs. Following MCMV intranasal infection, immune cells from nasal mucosa and lungs were isolated by fluorescence-acitivated cell sorting (FACS) and analyzed using scRNA seq and CITEseq.