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HPIV-1 Primer Information for Amplification and Sequencing.

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https://figshare.com/articles/dataset/_HPIV_1_Primer_Information_for_Amplification_and_Sequencing_/239145
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*Bold font represents oligonucleotides used for amplification. All primers were used for sequencing. The primer names are designed as follows: 1) The “P1” at the beginning indicates that these are HPIV-1 primers, 2) The “F” or “R” located at the third position of the primer name indicates whether the primer is in the forward or reverse orientation, 3) the number between the “F” or “R” and the hyphen corresponds to the nucleotide position of the 5′ end of the oligonucleotide in the HPIV-1 genome, and 4) adding or subtracting the number at the end of the primer name from the nucleotide position of the 5′ end of the oligonucleotide is the nucleotide position of the 3′ end of the oligonucleotide.
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2012-09-27
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