In situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling: Part2

E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was used to assess the false-positive rates in the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 22,000 DNA features, and was designed to detect transcripts from the entire NIA cDNA clone collection. Keywords: self vs self design E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "self-versus-self" experimental design was used to assess the false-positive rates in the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 22,000 DNA features, and was designed to detect transcripts from the entire NIA cDNA clone collection.收集3窝小鼠胚胎发育第12.5天（E12.5）的完整胚胎与胎盘并合并，随后提取总RNA。从上述RNA样本中制备经荧光标记、线性扩增的互补RNA（cRNA）靶标，采用新型微阵列设计开展杂交比对以验证该实验系统。本研究采用“自身与自身”实验设计，用以评估该系统的假阳性率。本次使用的原位合成60聚体寡核苷酸微阵列平台包含约22000个DNA探针特征，其设计用于检测美国国家衰老研究所（NIA）全套互补DNA（cDNA）克隆文库对应的转录本。关键词：自身与自身实验设计 收集3窝小鼠胚胎发育第12.5天（E12.5）的完整胚胎与胎盘并合并，随后提取总RNA。从上述RNA样本中制备经荧光标记、线性扩增的互补RNA（cRNA）靶标，采用新型微阵列设计开展杂交比对以验证该实验系统。本研究采用“自身与自身”实验设计，用以评估该系统的假阳性率。本次使用的原位合成60聚体寡核苷酸微阵列平台包含约22000个DNA探针特征，其设计用于检测美国国家衰老研究所（NIA）全套互补DNA（cDNA）克隆文库对应的转录本。