Expression data from HUVECs infected with influenza A virus PR8 and CA07. Homo sapiens

Using human umbilical vein endothelial cells (HUVECs) as cell models, the present study aims to explore the differential miRNAs in influenza virus-infected ECs and analyze their target genes involved in EC permeability regulation. As the results showed, permeability increased and F-actin cytoskeleton reorganized after HUVECs infected with influenza A virus (CA07 or PR8) at 30 MOI. most of these miRNAs were down-regulated after flu infection. It has been reported that PKC, Rho/ROCK, HRas/Raf/MEK/ERK, and Ca2+/CaM pathways are activated by flu infection and play important roles in permeability regulation. Overall design: When grown to 90% confluency, HUVECs were removed from the culture medium and washed twice with PBS. Cells were infected with influenza A virus in serum-free media at a multiplicity of infection (MOI) of 20~40 and harvested at the indicated time points. The non-infected cells were used as normal control.