Smart-seq2 analysis of P17 FACS sorted retinal cells from the Tg(Chx10-EGFP/cre,-ALPP)2Clc or Vsx2-GFP transgenic line

Three Vsx2-GFP mouse retinas were dissected, dissociated and FACS sorted, and single cell RNA-seq libraries were generated for 288 single cells and 3 bulk libraries using Smart-seq2 (~10,000 cells each) Three mouse retinas (labeled 1a, 2a, 2b) were used, and 96 single cells from each were processed using Smart-seq2. A bulk RNA-seq control of 10,000 cells from each retina was also prepared我们获取3例Vsx2-GFP小鼠视网膜，依次完成解剖分离、组织解离及FACS（荧光激活细胞分选）分选；随后采用Smart-seq2技术，为288个单细胞构建单细胞RNA测序文库，并为3个批量RNA测序文库制备样本，每个批量文库对应约10000个细胞。本实验所用的3例小鼠视网膜分别标记为1a、2a、2b，每例视网膜分离得到的96个单细胞均通过Smart-seq2技术完成处理。此外，我们还为每例视网膜的10000个细胞制备了批量RNA测序对照文库。