Additional file 2 of Cold adaptation and horizontal gene transfer shape Antarctic sponge microbiomes

Additional file 2: Supplementary Table S1. Supplementary Table S1. Metadata associated with the Antarctic, tropical, and temperate sponge microbiomes and Antarctic seawater microbiomes used in this study. Asterisks in "Sponge_status" indicate apparent classification of LMA sponges based on alpha diversity and phylum composition. Supplementary Table S2. Quantity and quality parameters of metagenomes (contigs, genes, annotations). Supplementary Table S3. List and counts of non-redundant genes classified into cold adaptation functions across all analyzed metagenomes. Supplementary Table S4. List and counts of non-redundant genes classified into the analyzed metabolic, resistance, and conjugative functions across all analyzed metagenomes. Supplementary Table S5. Pairwise PERMANOVA comparisons for the effect of the environment on the gene composition of microbiomes based on Bray–Curtis dissimilarities. Significance: "." indicates p < 0.05, "*" indicates p < 0.01, "**" indicates p ≤ 0.001. Supplementary Table S6. Gene proportions (%), abundance (RPKG), and diversity (Shannon index) of the functional groups analyzed in this study. The functional groups comprise cold adaptation, amino acid transport and metabolism (ATM), carbohydrate transport and metabolism (CTM), energy production and conversion (EPC), lipid transport and metabolism (LTM), metal and antibiotic resistance and metabolism (MAR), and machinery of integrative and conjugative elements (ICE). Supplementary Table S7. Taxonomy-assignment and quantity and quality parameters of MAGs (contigs, genes, annotations). Supplementary Table S8. Donor and recipient taxa of horizontally transferred functions for cold adaptation and the type of HGT event.