RNA-seq experiment of the amoeboflagellate Orciraptor agilis feeding on Mougeotia sp.. undefined

In this study transcriptomic data of three life history stages of Orciraptor agilis was generated: 1) Gliding cells in absence of food ("gliding"), 2) Cells attached to the cell wall of its algal prey during perforation ("attacking"), 3) Cells after acquisition of the algal plastid matieral ("digesting"). Furthermore, RNA-seq of the algal prey Mougeotia sp. was also performed. A de novo transcriptome assembly of the algal reads was performed in order to identify and substract algal reads of the Orciraptor samples by mapping the Orciraptor reads to the algal transcriptome. After this filtering step the remaining Orciraptor reads from all libraries were pooled for a de novo transcriptome assembly of Orciraptor agilis. This transcriptome was the basis for a comparative transcriptomic study in which transcript expression was compared between the three life history stages.