Comparison of percent off-target reads with varying protocol modifications.

P-values generated from pairwise t-tests comparing the percentage of the total reads that were not associated with a Twist target probe (within +/- 200 bp) for each capture efficiency experiment. 65C/68C refers to annealing temperature and 0uL/2uL/4uL ME refers to volume of methylation enhancer. * represents a significant (p < 0.05) difference in the percent of probes captured between conditions. (XLSX)