Potential Target Genes of MicroRNA-21 In Ischemia-Reperfusion Injury Of The Gracilis Muscle In Rats

Following 4 h of ischemia and subsequent reperfusion for 4 h of the gracilis muscles, three miRNAs (miR-21, miR-200c, and miR-205) of 350 tested rat miRNAs were found to have an increased expression in the miRNA array.The expression of the mRNA in the muscle specimens after 4 h of ischemia and reperfusion for 1, 3, 7, and 14 d were detected with the Agilent Whole Rat Genome 4 × 44 k oligo microarray. A combined approach using a computational prediction algorithm that included miRanda, PicTar, TargetScanS, MirTarget2, RNAhybrid, and the whole genome microarray experiment was performed by monitoring the mRNA:miRNA association to identify potential target genes.对股薄肌（gracilis muscle）施以4小时缺血及后续4小时再灌注处理后，在350个被测大鼠微小RNA（miRNA）中，有3种miRNA（miR-21、miR-200c与miR-205）经miRNA芯片检测发现表达量上调。在缺血再灌注处理后1、3、7及14天采集的肌肉标本中，信使RNA（mRNA）的表达水平通过安捷伦全大鼠基因组4×44k寡核苷酸微阵列完成检测。本研究采用联合分析策略：运用涵盖miRanda、PicTar、TargetScanS、MirTarget2及RNAhybrid在内的计算预测算法，并结合全基因组微阵列实验，通过监测mRNA与miRNA的结合互作关系以鉴定潜在靶基因。