AEC II cell differentiation in miR142 KO mice

We observed that loss of miR142 leads to the increased expression of surfactant protein C and decreased Podoplanin.This indicates increased differentiation of alveolar progenitors towards AEC II lineage. In order to understand 1) what are the genes that are being regulated during AEC II cell differentiation in miR142KO mice we are performing this experiment. Two groups (wild type and mir142 knock-out mice), n=4 mice per group我们观察到，miR142缺失会导致表面活性蛋白C（surfactant protein C）表达上调，同时使平足蛋白（Podoplanin）的表达水平降低。该结果提示肺泡祖细胞向II型肺泡上皮细胞（alveolar epithelial cell type II，AEC II）谱系的分化程度增强。为了明确miR142基因敲除（miR142 knockout，miR142KO）小鼠体内，AEC II细胞分化过程中受调控的基因有哪些，我们开展了本项实验。实验分为两组：野生型小鼠与miR142基因敲除小鼠，每组包含4只小鼠。