RNA immunoprecipitation and sequencing of aminoacyl-tRNA synthetase-specific genes

To gain insight into the specificity of target mRNAs, we enriched mRNAs bound to TRS by immunoprecipitation with anti-TRS antibody, then performed RNA immunoprecipitation and sequencing (RIP-seq) and compared the mRNAs with those enriched by immunoprecipitation with IgG, anti-AlaRS, anti-PRS and anti-IRS antibodies. Examination of ARS-specific mRNAs by RIP-seq experiments by using specific TRS, AlaRS, IRS, and PRS antibodies