16s rDNA sequencing of feces from C9orf72 loss of function mice

We report the sequencing of bacterial species across four environments in which C9orf72 loss of function mice were studied as well as mice that received fecal transplantation. Our study elucidates bacterial communities that associate with pro-inflammatory or pro-survival outcomes in this model of ALS/FTD with features of autoimmunity and systemic and neural inflammation. Overall design: In one round of sequencing, 5 fecal pellets from 2 pro-inflammatory environments (Harvard BRI/Johns Hopkins) and 2 pro-survival environments (Broad Institute/Jackson Labs) were sequenced at the 16s rDNA locus. In a second round of sequencing, 9 fecal pellets from Harvard BRI, 9 fecal pellets from Broad Institute, 6 fecal pellets from Harvard BRI mice transplanted with Harvard BRI feces, and 6 pellets from Harvard BRI mice transplanted with Broad feces were sequenced at the 16S rDNA locus.