NGS- IL-12 treated primary murine neuronal cultures

We performed next generation sequencing of primary murine neurons stimulated with recombinant IL-12 to test the isolated effect of IL-12 on neurons, independent from a heavily inflamed CNS microenvironment. Principal component analysis (PCA) revealed a dose-dependent response to IL-12 in the neuronal transcriptome. Our computational analysis identified 1,878 significantly DEGs (p≤0.01, log ratio ≥0.5). Several of the upregulated genes have been linked to promoting neurogenesis (Fpr1, Mbdi1), neuroprotection (Timp1, Angptl4) and enhancing remyelination (Timp1, Matn2), thereby suggesting a direct neuroprotective and neurotrophic effect of IL-12.

Sequencing raw data and processed gene expression data will be deposited into the GEO repository upon publication. Code for analyses will be available upon contacting the corresponding authors: [e-mail] and [e-mail]

Sequencing raw data and processed gene expression data will be deposited into the GEO repository upon publication.