Systematic affinity purification mass spectrometry (AP-MS) reveals LCMV interacted human proteins
收藏国家人口健康科学数据中心2026-06-01 收录
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https://www.ncmi.cn/phda/dataDetails.do?id=CSTR:17970.11.A001G.202308.2746.V1.0
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资源简介：
To systematically map the arenavirus‒host protein interaction, we employed a systematic affinity purification mass spectrometry (AP-MS) approach to define the human proteins that physically interact with LCMV proteins. The four LCMV proteins (NP, LP, GPC, Z) and control protein GFP were tandemly cloned with Twin-Strep tags at C-terminal based on the same backbone pCAGGs vector respectively. Proteins were expressed in HEK293T cells followed by affinity-purification based on Strep-Tactin System. We summarized the number of human proteins interacting with each indicated LCMV protein baits and identified 284 human proteins with high-confidence PPIs (log2 enriched fold > 1, P < 0.05).
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北京蛋白质组研究中心创建时间：
2023-07-28
