Supplementary file
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Fig. S1. Maximum likelihood tree of red algae based on the NADPH-oxidase (Rboh) protein sequences. Numbers on branches are ML bootstrap % (BP) values. Grey bar represents outgroup, bacterial ferric reductase. Apricot bar represents unicellular red algal Rboh lineage. Pink bar represents Florideophyceae Rboh lineage 1-4. Scale bar = substitutions per site.Fig. S2. Range of real-time polymerase chain reaction cycle threshold (Ct) values of candidate housekeeping genes on different days after infection of Dasysiphonia japonica. A-tubulin: alpha-tubulin, GAPDH: glyceraldehyde 3-phosphate dehydrogenase, EF-Tu: elongation factor thermos unstable, IDH: isocitrate dehydrogenase, UBI: ubiquitin-conjugating enzyme.Video S1. Live time video of Olpidiopsis heterosiphoniae zoospores released from discharge tube.Video S2. Live time video of Olpidiopsis dasysiphoniae zoospores released from multiple discharge tube.
