Percentage of cell viability in AsPC-1, HUVECs and WI-38 cells exposed to Gem, Bev, Su and EMAP, either alone or in combination.

Cells were seeded into 96-well plates and treated with Gem, Bev, Su and EMAP. A represents 100 nM for Gem, Su, E and 1 µg/ml for Bev; B represents 500 nM for Gem, Su, E and 10 µg/ml for Bev; C represents 1 µM for Gem, Su, E and 100 µg/ml for Bev; D represents 10 µM for Gem, Su, E and 1000 µg/ml for Bev. After 72 hours incubation, 10 µl WST-1 reagent was added to each well, and absorbance of color produced was measured at 450 nm that correlates with the number of viable cells in the well. Data are expressed as the mean value ± standard deviation of quadruplicate determinants.