LANA and KAP1 ChIP-seq in BC3. Human gammaherpesvirus 8

Kaposi’s sarcoma-associated herpesvirus (KSHV) typically displays two different phases in its life cycle, including the default latent phase as well as the lytic phase. There is a short period of lytic gene expression at the early stage of KSHV primary infection. It has been shown that the latency-associated nuclear antigen (LANA) encoded by KSHV plays an important role in the establishment of viral latency. In screening, we identified a host protein, Krüppel-associated box domain associated protein-1 (KAP1) that bound to LANA. We proved that LANA recruited KAP1 to the RTA promoter region of the KSHV genome. To determine whether LANA recruited KAP1 to other regions of the KSHV genome, we mapped the whole genome-wide binding sites of LANA and KAP1 by ChIP-seq (the viral and the host genome).