Effect of genistein, AMPK activator, and AMPK inhibitor on TNF-α and IL-6 production.

LPS+AICAR: cells were pretreated with 1 mM AICAR for 1 h and then incubated with 1 µg/mL LPS for 24 h; LPS+Gen 10 µM: cells were pretreated with 10 µM genistein for 1 h and then incubated with LPS for 24 h; LPS+Com C: cells were pretreated with 20 µM Compound C for 30 min and then incubated with LPS for 24 h; LPS+Gen 10 µM+Com C: cells were pretreated with 20 µM Compound C for 30 min, incubated with 10 µM genistein for 1 h, and then co-cultured with LPS for 24 h. Values are mean ± S.E.M of three independent experiments.*P **P#P##P△P△P