Dataset for: Validation of a routine two-sample iohexol plasma clearance assessment of GFR and an evaluation of common endogenous markers in a rat model of CKD

Endogenous markers of kidney function are insensitive to early declines in GFR and in rodent models,
validated, practical alternatives are unavailable. In this study we determined GFR by modelling the
plasma clearance of two compounds, iohexol and inulin, and compared the findings to common
endogenous markers. All plasma clearance methods of both iohexol and inulin detected a decline in renal
function weeks prior to any increase in endogenous marker. Iohexol plasma clearance and inulin plasma
clearance had very high agreement and minimal bias when using 12-sample models. However, only
iohexol could be accurately simplified to a two-sample, 1-compartment estimation strategy. Following an
IV injection of low-dose iohexol and two timed blood samples at 30 and 90 minutes, one can accurately
approximate a complex 12-sample strategy of plasma clearance. This method is simple enough to use in
routine, longitudinal analysis of larger cohort animal studies.