Differentiation of two Colletotrichum lentis races based on repeat variations of a 39 nt minisatellite in IGS.

* Isolates published by Buchwaldt et al. (2004).# Used for production of additional single spore isolates.SK = Saskatchewan, MB = Manitoba. tef1α = translation elongation factor 1α, rpb2 = RNA polymerase II subunit B2, acla = ATP citrate lyase subunit A.The IGS, ITS and three genes, tef1α, rpb2 and acla were sequenced in isolates representative of the two races. A probe, 39F/R, amplified 7 and 9 of the 39 nt repeats in race Ct1 isolates, while race Ct0 isolates had from 2 to 12 repeats, but never 7 or 9. The number of the 39 nt repeat was determined by gel electrophoresis and verified by sequencing of DNA extracted from gel bands.