Next Generation Sequencing Facilitates Quantitative Analysis of mRNA expression of STAT3 deficiency skin

Dominant-negative mutations of signal transducer and activator of transcription 3 (STAT3) signaling which controls epithelial proliferation in various tissues, leads to atopic dermatitis (AD) in hyper IgE syndrome (HIES). We found that the STAT3 signaling in keratinocytes is required to maintain skin homeostasis through this transcriptome analysis. Ear skin of skin specific-Stat3 sufficient or deficient mice were taken and analyzed the transcriptome using Illumina HiSeq1500.调控多种组织上皮细胞增殖的信号转导与转录激活因子3（signal transducer and activator of transcription 3, STAT3）信号通路的显性负突变，可在高IgE综合征（hyper IgE syndrome, HIES）中诱发特应性皮炎（atopic dermatitis, AD）。本研究通过本次转录组分析证实，角质形成细胞（keratinocytes）中的STAT3信号通路是维持皮肤稳态的必需条件。我们采集了皮肤特异性Stat3功能正常或缺陷的小鼠耳部皮肤样本，采用Illumina HiSeq1500平台完成转录组测序与分析。