Gene expression data of nasopharyngeal carcinoma (NPC) cell and Thiostrepton treatment

Thiostrepton, a natural antibiotic, has recently been shown to be a potential anticancer drug for certain cancers, but its study in nasopharyngeal carcinoma (NPC) is still limited. The aims of this study were to investigate the anticancer effect of thiostrepton on NPC cells and to explore its underlying mechanism. Our results showed that thiostrepton reduced NPC cell viability in a dose-dependent manner. Thiostrepton inhibited the migration and invasion of NPC cells in wound healing and cell invasion assays. Thiostrepton effectively suppressed NPC cell proliferation, migration, and invasion, likely by several mechanisms. Thiostrepton may be a potential therapeutic agent for treating NPC in the future. Total RNA from NPC cells treated with or without 4 μM thiostrepton for 12 hours at 37°C was extracted using an illustra RNAspin Mini RNA isolation kit (GE Healthcare, Little Chalfont, UK) according to the manufacturer’s instructions. Amplification and biotinylation of the RNA and hybridization of the fragmented biotin-labeled cRNA with Affymetrix HG-U133 Plus 2.0 GeneChip microarrays.