The impact of SpiB overexpression on gene expression profile in A549 cells

SpiB, an Ets family transcription factor, is expressed exclusively in mature B cells, T-cell progenitors, and plasmacytoid dendritic cells. To understand the genes regulated by SpiB in epithelial cells, we overexpressed SpiB in lung cancer cell line A549 and examined the change of transcription profile using Agilent SurePrint G3 Human Gene Expression 8x60K v2 Microarray (Agilent Technologies). Two-condition experiment, A549-empty vs. A549-SpiB cells. Biological replicates: 3 control, 3 transfected, independently grown and harvested. One replicate per array.