Single cell RNA-seq analysis of the Rbpj mutant mouse kidney by Foxd1Cre

The goal of our study is to determine if interstitial Notch signaling is required for the formation/development of nephron segments. Rbpj flox allele was deleted in the mouse renal interstitium using Foxd1Cre. Mouse kidneys at P0 were collected and subjected to scRNA-seq analysis. Examination of four groups: two controls (Rbpj c/+ ; Foxd1cre/+) and two mutants (Rbpj c/- ; Foxd1cre/+)本研究旨在明确间质Notch信号通路是否为肾单位节段的形成与发育所必需。研究通过Foxd1Cre工具在小鼠肾间质中敲除Rbpj flox等位基因，收集出生后0日龄（P0）的小鼠肾脏并开展单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）分析。实验共设置四组样本：两组对照组（Rbpj c/+ ; Foxd1cre/+）与两组突变组（Rbpj c/- ; Foxd1cre/+）