Identification of significantly differential expression of long noncoding RNAs and pathways in hepatic fibrosis rat liver issues

Objective: To identify the long noncoding (lncRNA) and its potential roles in hepatic fibrosis rat liver issues induced by CCl4. Methods: LncRNAs and genes were analyzed in fibrosis rat liver issues by RNA sequencing and verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Differential expressed lncRNAs and genes were subjected to bioinformatics analysis and involved to construct co-expression network.研究目的：鉴定四氯化碳（CCl4）诱导的肝纤维化大鼠肝组织中的长链非编码RNA（long noncoding RNA，lncRNA）及其潜在作用。方法：采用RNA测序技术对纤维化大鼠肝组织中的lncRNA与基因进行分析，并通过定量反转录聚合酶链反应（quantitative reverse transcription polymerase chain reaction，qRT-PCR）完成验证；对差异表达的lncRNA与基因开展生物信息学分析，并构建共表达网络。