Transcriptome sequencing of two Populus species

We conducted a micro-grafting experiment by reciprocally grafting young scions to young rootstocks between two Populus species, P. szechuanica var. tibetica (S) and P. tomentosa Carr. (T) from two different sections of the Populus genera, to form four grafting combinations, S/S, S/T, T/S, and T/T. Stems of scions and rootstocks were collected for transcriptomic measurements at 8 time points, 6, 8, 12, 18, 26, 36 and 48 DAG (days after grafting). These samples named after scion species-rootstock species-sample position-sampling time; e.g., "STR38" represents "the rootstock of P. szechuanica var. tibetica /P. tomentosa, the 3rd replicate, 8 DAG".