DNA-RNA IP sequencing for R-loops in XPO4 Knockout NIH/3T3 cell. DNA-RNA IP sequencing for R-loops in XPO4 Knockout NIH/3T3 cell

Numerous RNAs are exported from the nucleus, abnormalities of which lead to cellular complications and diseases. We report the identification of Exportin 4 (XPO4) as a conserved circular RNA (circRNA) exportin. Cellular insufficiency of XPO4 causes nuclear circRNA accumulation, circRNA:DNA (ciR-loop) formation, linear RNA:DNA (liR-loop) buildup, and DNA damage. These results provide insights into the mechanism and biomedical links of circRNA nuclear export. Overall design: Examination of R-loops level in NIH/3T3 cells.