Genome assembly (Flye) of Biomphalaria sudanica Bs2280 PacBio Revio HiFi reads
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High molecular weight DNA from a single snail included in the pooled-GWAS (Bs2280) that was phenotypically resistant (not shedding cercariae or S. mansoni PCR positive) and contained non-reference (resistant) haplotypes assessed using the amplicon panel at both SudRes1 (contigs c582_65596: TT, contig_6_3490: AT and contig_6844_72816: GT, the latter two not indicating heterozygosity but rather paralogs, since there are never any TT’s) and SudRes2 loci (contig sc94 positions 2174117: AA, 2150435: CC and 2149367: AA) was sequenced on the PacBio platform. Genomic DNA was cleaned, fragmented and library produced following the standard HiFi SMRTbell Library preparation by Novogene (Sacramento, CA). The library was sequenced on a single PacBio Revio using a single SMRT cell.
创建时间:
2025-03-24



