Computer Simulation of SNP Accrual in the Setting of a Transition Bias Leads to Enhancement of Conservative Mutations.

SNPs were stochastically placed in 1) an artificial, assembled gene containing 1480 codons arranged randomly (i.e. random codons were used to generate a 4440 bp sequence), 2) the CFTR coding sequence (1480 codons), or 3) a GC-rich region of CFTR. The computer-generated positions to be mutated were selected randomly, and the choice of base replacement (e.g. with or without a particular transition bias) derived as above, according to the CFTR mutation database (Table 2), or rates observed for exonic or intronic SNPs (Table 1). The ratios for non-conservative (Ncon) to conservative (Con) SNPs are shown. Table 5 is the result of 10 simulation runs per sequence, indicating significant differences even after small numbers of SNP incorporation.Computer Simulation of SNP Accrual in the Setting of a Transition Bias Leads to Enhancement of Conservative Mutations.