Single cell RNA sequencing of lung dendritic cells in healthy and hyperglycemic mice. Single cell RNA sequencing of lung dendritic cells in healthy and hyperglycemic mice

We performed single cell RNAseq to measure gene expression in different subsets of lung dendritic cells hyperglycaemic mice. We used streptozotocin model to achieve hyperglycaemia. Each sample contains cells from 4 different mice and hashing was done to allow disentangling which cell comes from which condition. Hashing was done with biolegend antibodies: Sample1: B0303: control 1, B0304 control 2, B0305 STZ 1, B0306 STZ 2 Sample2: B0303: control 3, B0304 control 4, B0305 STZ 3, B0306 STZ 4