Results of single pair crosses of individuals of inferred male-killer status and suppressor gene genotype.

The expected sex ratio is based on the suppressor gene being a normal Mendelian locus, with the suppressor allele (res+) dominant to the non-suppressor allele (res−), and on a vertical transmission efficiency ranging from 0.8–1. The ranges of uncertainty indicate the 95% confidence limits of the expected sex ratios, incorporating binomial sampling and calculated by simulation. All sex ratios are given as proportion male. The generation of the maternal parent in a cross is indicated by number of full stops + 1. * indicates that the individual used was offspring of a second male mated to the Mk1.1 female. See Table S2 for further detail of the matrilines. All females were shown, by post hoc sequence analysis, to carry the male-killer.