Metadynamics production runs were conducted using GROMACS 2023.2 patched with PLUMED-2.9.0, incorporating the RBB-NA code available on GitHub. The RBB-NA algorithm enables control of rigid base parame
Activation of gene transcription in eukaryotes requires the cooperative assembly of an initiation complex containing many protein subunits. The necessity that these components contact each other and t
